Regulatory

Part:BBa_K2771002

Designed by: Felipe Xavier Buson   Group: iGEM18_USP-Brazil   (2018-10-06)


Modified pTra

Quorum sensing activated promoter that responds natively to the Tra pathway (activated by TraR + 3OC8HSL). This specific instance of this promoter has a diversity of differences to the wild-type promoter, sharing a consensus between bases 2 and 16. The sequence was optimised by SR. Scott and J. Hasty (doi: 10.1021/acssynbio.5b00286) to show diminished crosstalk with the Lux, Las and Rpa systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Contribution of SCAU-China

We use it to construct our three-tier cascading regulatory circuit based on two orthogonal quorum-sensing systems.[1]


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Categories
Parameters
None